南方农业学报2011，Vol.42Issue(10)：1185-1188,4.
荸荠试管苗壮苗培育及小球茎再生研究
Cultivation of vigorous plantlets and corm production from water chestnut test tube plantlets
摘要
Abstract
[Objective]The aim of the experiment was to develop a method for obtaining vigorous test tube plantlets of water chestnut (Eleocharis tuberosa ), and to find out the best induction condition for their conns. [Method]The test tube plantlets of water chestnut variety Guiti 1 were used to study the effects of different concentrations of sucrose (30.0-120.0 g/L), PP333 (0-1.0 mg/L) and temperatures on culturing vigorous test tube plantlets and reproducing corm. [Result]Addition of 1.0 mg/L 6-BA in culture medium containing 30.0-120.0 g/L sucrose resulted in induction of small corms in test tube plantlet. The best induction effects were observed at 30.0 g/L concentration of sucrose, over which the induction of corms was found to be inhibited. Addition of 2.5 mg/L 6-BA in culture medium resulted only in production of stolon. Similarly, addition of NAA, IBA and different concentrations of sucrose in culture medium resulted in development of stolon, but the corm induction from stolon occurred only in medium containing 30.0-60.0 g/L, and the treatment with 30.0 g/L sucrose was found most effective. Addition of NAA, IBA and different concentrations of PP333(0.3-1.0 mg/L) in culture medium resulted in induction of vigorous corm and roots, however quantity of induced corms did not differ in different treatments. Under culture temperature of 15-20℃ and adding auxin and lower 6-BA in medium, the plantlet could not induce the corm in all media adding sucrose. The corms could be induced in medium with lower sucrose under conditions of 28-30℃ for 20 days before culture and 15-26℃ for 40 days after culture. [Conclusion]The MS culture medium containing 0.5 mg/L NAA+ 0.5 mg/L IBA + 30.0 g/L sucrose + 3.5 g/L agar supplemented with 0.3-1.0 mg/L PP333 showed the best effects on culturing vigorous plantlet. Adding suitable 6-BA or NAA and IBA or sucrose in culture medium could induce corm of plantlet.关键词
荸荠/试管苗/壮苗/球茎/诱导Key words
water chestnut (Eleocharis tuberosa)/ tissue culture/ vigorous plantlet/ corm/ induction分类
农业科技引用本文复制引用
潘颖南,杭玲,陈丽娟,张向军..荸荠试管苗壮苗培育及小球茎再生研究[J].南方农业学报,2011,42(10):1185-1188,4.基金项目
广西科技攻关项目(桂科攻10100008-5) （桂科攻10100008-5）
