南方农业学报2011，Vol.42Issue(11)：1307-1310,4.
香蕉突脐蠕孢叶斑病菌Brn1基因的PCR扩增与序列分析
PCR amplification and sequence analysis of Brn1 gene of Exserohilum rostratum causing banana leaf spot disease
摘要
Abstract
[Objective]Different strains of Exserohilum rostratum,causing exserohilum banana leaf spot disease were characterized at molecular level using the conserved sequence of Brn1 gene.[Method] Brn1 gene of three pathogenic isolates (CLER09,D087 and JL05) of E.rostratum causing exserohilum banana leaf spot disease was amplified using primer pair Brn1 01 (5'-GCCAACATCGCAAACATGG-3') and Brn1 02 (5'-GCAAGCAGCACCGTCAATACCAAT-3'),and phylogenetic analysis was carried out.[Result] The results showed absence and substitute of single bases in Brn1 sequences among different isolates of E.rostratum isolated from diseased banana plant and sequence collected from GenBank database.The isolates collected from banana were clustered with E.rostratum in an absolute evolution group in the phylogenetic tree,and significantly distinguished from other species of Exserohilum spp.Analysis result of the genetic distance matrix showed that tested isolates were genetically close to E.rostratum sequences collected from GenBank database with intraspecies genetic distance of 0.000-0.018 and interspecies genetic distance of 0.020-0.093 in Exserohilum genus.[ Conclusion ] The obvious genetic difference among isolates of E.rostratum could be considered as intraspecific variation and the Brn1 gene sequence may be used for molecular evidence for supporting the isolates with markedly morphological difference as members of E.rostratum.关键词
嘴突脐蠕孢/系统发育/Brn1降解酶基因/序列分析Key words
Exserohilum rostratum/ phylogeny / Brn1 reductase gene/ sequence analysis分类
农业科技引用本文复制引用
林善海,黄思良,覃丽萍,胡春锦,余功明,付岗..香蕉突脐蠕孢叶斑病菌Brn1基因的PCR扩增与序列分析[J].南方农业学报,2011,42(11):1307-1310,4.基金项目
Guangxi Science Foundation Project (No.0728071) （No.0728071）
