南方农业学报2011，Vol.42Issue(11)：1320-1323,4.
多效唑、矮壮素对彩色马蹄莲试管微型种球诱导的影响
Effects of paclobutrazol and chlorcholine chloride on induction of micro-tube seed bulbs of Zantedeschia hybrida
摘要
Abstract
[Objective]The present experiment was conducted to induce corm in test tube plantlet derived from Zantedeschia hybrida to enhance propagation efficiency and transplant survival rate, shorten seedling raising lime, reduce occurrence of serious disease and decrease high production cost. [ Method ]The test tube plantlets with 3-7 cm height and 0.3 cm diameter were used asexplants and inoculated to culture medium containing different sugar (2-8%), paelobutrazol (1 -8 mg/L) and chlorcholine chloride (2-16 mg/L), the formation rate of corm was calculated after culturing for 90 days. [ Result ]The formation rate of corm in Zantedeschia hybrida showed an increasing trend with increase in sugar in improved MS medium adding same paclobutrazol and chlorcholine chloride. With the increase of paclobutrazol and chlorcholine chloride in medium, the formation rate of corm increased initially, followed by a decrease. The induction effects of paclobutrazol (4 mg/L) and chlorcholine chloride (8 mg/L) was found the best. The highest corm formation rate was achieved in medium containing 4 mg/L paclobutrazol and 6% sugar. This combination also resulted in best average diameter and fresh weight of corm. The paclobutrazol and chlorcholine chloride had inhibitory effect on the growth of leaf, and differentiation and elongation of root [Conclusion]Adding suitable rate of sugar and paclobutrazol or chlorcholine chloride in improved MS+NAA (0.1 mg/L)+ agar (7 g/L) solid medium, can successfully induce the formation of corm in Zantedeschia hyhrida, and the induction effect of paclobutrazol was better than that of chlorcholine chloride.关键词
彩色马蹄莲/微型种球/诱导/多效唑/矮壮素/蔗糖Key words
Zantedeschia hybrida/ micro seed bulb/ induction/ paclobutrazol/ chlorcholine chloride/ sucrose分类
农业科技引用本文复制引用
张翠萍,赵健,唐凤鸾,仇硕,李秀娟..多效唑、矮壮素对彩色马蹄莲试管微型种球诱导的影响[J].南方农业学报,2011,42(11):1320-1323,4.基金项目
广西科学基金项目(0991225) （0991225）
桂林市科技成果转化与应用项目(20090107-6,20100119) （20090107-6,20100119）
广西植物研究所科研基金项目(桂植业10010,桂植业09029) （桂植业10010,桂植业09029）
