南方农业学报2011，Vol.42Issue(11)：1395-1398,4.
蓝莓叶中可萃取多酚和不可萃取多酚提取的研究
Analysis on extractable and non-extractable polyphenols in blueberry leaves
摘要
Abstract
[Objective]The present experiment was conducted to study the effects of solvent type, volume and pH on the content of extractable polyphenols and non-extractable polyphenols in blueberry leaves to provide basis for commercial extraction of polyphenois from blueberry leaves. [ Method ] The leaf of blueberry was used to extract the extractable polyphenol using two-step extraction method. The non-extractable polyphenols in leaves of blueberry were isolated by sulfuric acid hydrolysis, and both polyphenol contents were determined by Folin-Cioealteu colorimetrie method. [Results] The results showed that the content of total extractable polyphenol (TEPP) was the highest under condition of 50:50 extrac-tanl to H2O ratio and pH 2 irrespective of the extractant used (carbinol or ethanol), while the non-extractable polyphenol (NKPP), on the contrary, showed lowest content. Therefore, low pH value and 50:50 extractant to H2O ratio and pH 2 were beneficial for extraction of TEPP, and not for NEPP. The content of total polyphenols in different treatments differed little and was over 82.00 mg/g. The analysis showed that the content of extractable polyphenol on the 1st step was mainly affected by pH value, while that content on 2nd step was affected significantly by single factor and complex factors, and these factors had no significant effects on the extraction of total polyphenol. The complex factors also have no significant effects on non-extractable polyphenol. [ Conclusion ]The pH value of extractant has significant effect on extraction of polyphenols, and the content of extractable polyphenol was similar to that of non-extractable polyphenols in the leaf of blueberry. The average total polyphenol content was recorded as 85.49 mg/g.关键词
蓝莓叶/可萃取多酚/不可萃取多酚/提取/萃取溶剂Key words
blueberry leaf/ extractable polyphenols/ non-extractable polyphenol分类
医药卫生引用本文复制引用
程安玮,杜方岭,王文亮,陶海腾,刘丽娜,徐同成..蓝莓叶中可萃取多酚和不可萃取多酚提取的研究[J].南方农业学报,2011,42(11):1395-1398,4.基金项目
国家青年科学基金项目(31101270) （31101270）
