南方农业学报2011，Vol.42Issue(11)：1423-1426,4.
食蟹猴精液低温保存研究
Preservation of Macaca fasicularis sperm under low temperature
摘要
Abstract
[Objective]The present experiment was conducted to obtain the best formulation for preserving Macaca fasicularis sperm to provide basis for its cryo-preservation and quality identification. [ Method] The survival time and survival index of Macaca fasicularis sperm were determined under three temperature conditions, viz., physiological (37℃),room(15-25℃ )and low(0-5℃)temperatures. [Result]The survival time of sperm preserved in TALP solution at 37 and 15-25℃ was 12.94±5.57 and 58.59±40.91 h, respectively, and the survival index of them was 4.88±1.97 and 24.48± 17.94, respectively. The survival time and index of sperm preserved in TALP solution added with 20% egg yolk at low temperature (0-5℃) were 596.00±83.35 and 283.05±49.42 h, respectively, which were significantly higher than those of other treatments (P<0.01). [ Conclusion ]The best formulation for preservation of Macaca fasicularis sperm was recorded as TRIS solution added with 20% egg yolk at 0-5℃.关键词
食蟹猴精液/低温保存/配方/精子/存活时间/存活指数Key words
Macaca fasicularis/ preservation at low temperature/ formulation/ sperm/ survival time/ survival index分类
农业科技引用本文复制引用
黎宗强,曾素先..食蟹猴精液低温保存研究[J].南方农业学报,2011,42(11):1423-1426,4.基金项目
广西自然科学基金项目(桂科自0991045) （桂科自0991045）
