南方农业学报2011，Vol.42Issue(12)：1476-1480,5.
水稻条纹病毒外壳蛋白叶绿体离体跨膜运输研究
Studies on in vitro rice stripe virus coat protein import into chloroplasts
摘要
Abstract
[Objective]The present experiment was aimed to find out the possibilities of rice stripe virus coat protein (RSV-CP) import into chloroplasts in vitro in order to provide references for studying the pathogenesis of rice stripe virus. [Method]Rice and wheat chloroplast was obtained by using leaf-cut method. The system of RSV-CP import into chloroplasts in vitro, and the effects of the incubation time and the concentration of RSV on import efficiency were also studied. [Result] RSV-CP was found able to import into isolated chloroplasts of RSV host plants including rice and wheat in 5 min. The lowest concentration of RSV in the import system was 58.1 jJig/mL and the best incubation time was 15 min in the current studies. By extending the incubation time or increasing RSV concentration, the amount of RSV-CP import into the chloroplasts was increased. [Conclusion]The study reveals that RSV-CP import into rice and wheat chloroplast in vitro is possible. This may be one of the leading causes of mosaic symtoms.关键词
水稻条纹病毒/外壳蛋白/叶绿体/跨膜运输Key words
rice stripe virus/ coat protein/ chloroplast/ transmembrane transport分类
农业科技引用本文复制引用
程兆榜,於春,任春梅,周益军,范永坚,谢联辉..水稻条纹病毒外壳蛋白叶绿体离体跨膜运输研究[J].南方农业学报,2011,42(12):1476-1480,5.基金项目
国家公益性(农业)行业专项项目(201003031) （农业）
江苏省自然科学基金项目(BK2010018) （BK2010018）
