广西师范大学学报（自然科学版）2011，Vol.29Issue(4)：104-110,7.
荔枝类黄酮糖基转移酶(UFGT)基因的克隆及其原核表达研究
Cloning Glucose-flavonoid 3-o-glucosyltransferase (UFGT) from Litchi and Expression in Escherichia coli
摘要
Abstract
Litchi is native to South China's tropical fruit trees,the fruit color is various types. Function of Glucose-flavonoid 3-o-glucosyltransferase (UFGT) is catalyzed the unstable anthocyanidin into antho-cyanin. It is the last enzyme in anthocyanin biosynthetic pathway. Within a certain range,UFGT activity is positively related to anthocyanin synthesis,and enzyme activity of UFGT inhibited has more influence on anthocyanin synthesis than other enzymes. Many research results show that UFGT is a key enzyme gene in anthocyanin synthesis. This study is based on the known UFGT gene fragments in the Litchi,the full-length cDNA of UFGT was cloned using the 3'RACE and 5'RACE methods. It was found an intron in the genomic DNA sequence compared to cDNA sequence. The cDNA fragment of UFGT directionally cloned into the vector pET32a,the UFGT fusion protein about 60 Kda was expressed by induction 1 mmol/L IPTG in E. coli.关键词
荔枝/花色素苷/类黄酮糖基转移酶/基因克隆/原核表达Key words
Litchi chinensis Sonn. /anthocyanin/ UFGT/ gene cloning/prokaryotic expression分类
农业科技引用本文复制引用
赵志常,胡福初,胡桂兵,王惠聪,杨转英,苏纯兰,李加强..荔枝类黄酮糖基转移酶(UFGT)基因的克隆及其原核表达研究[J].广西师范大学学报（自然科学版）,2011,29(4):104-110,7.基金项目
国家科技支撑计划(2006BAD01A1705) （2006BAD01A1705）
现代农业产业技术体系建设专项资金资助项目(nycytx-32) （nycytx-32）
国家自然科学基金资助项目(30971985) （30971985）
