广西医科大学学报2011，Vol.28Issue(6)：830-833,4.
TGF-β1、TNF-α及羟脯胺酸在大鼠放射性肺损伤中的变化及意义
CHANGES AND SIGNIFICANES OF TGF-β1, TNF-α AND HYDROXYPROLINE IN RADIATION-INDUCED PULMONARY INJURY
摘要
Abstract
Objective: To investigate the possible mechanisms of radiation-induced lung fibrosis. Methods: The right hemithorax of SD rats were irradiated with a single dose of 7. 0 Gy and 14. 4 Gy. All animals were sacrificed and the lungs were removed at 1, 7, 30 and 90 days after radiation. The histopathological changes, hydroxyproline content and expression of transforming growth factor-β1 (TGF-β1 ) , tumor necrosis fac-tor-α (TNF-α) between the radiation and control groups were analyzed by HE staining. Masson staining and immunohistochemistry. Results:In control group and 7. 0 Gy groups, there were no notable histopathological changes, just including little tissue exudates, congestion, edema and infiltration of inflammatory cells, red blood cell leakage. However, the formation of collagen and fibrosis could be observed in 14. 4 Gy group. The expression of TGF-β1 , TNF-a was negative or weak positive in the control group and radiation groups. But, both them were increased in 14. 4 Gy group. The content of hydroxyproline was little changes in the control and 7. 0 Gy groups at every time point, it increased at 30 days significantly, and up to maximum at 90 days in 14. 4 Gy group after radiation. Conclusion: Radiation-induced lung injury in the inflammatory response stimulated radiation lung injury and fibrosis process.关键词
放射性肺损伤/转化生长因子/肿瘤坏死因子/羟脯胺酸Key words
radiation injury/ transforming growth factor-β1 / tumor necrosis factor-α/ hydroxyproline分类
医药卫生引用本文复制引用
何天楚,李坚,邱俊..TGF-β1、TNF-α及羟脯胺酸在大鼠放射性肺损伤中的变化及意义[J].广西医科大学学报,2011,28(6):830-833,4.基金项目
广西自然科学基金资助项目(No.Z305519) （No.Z305519）
