广西医科大学学报2011，Vol.28Issue(6)：834-836,3.
慢病毒载体介导RNA干扰对乳腺癌MDA-MB-231细胞LOX基因表达的影响
EFFECTS OF LOX GENE EXPRESSION ON BREAST CANCER CELL LINE MDA-MB-231 BY LENTIVIRAL VECTOR-MEDIATED RNA INTERFERENCE
摘要
Abstract
Objective:To explore lysyl oxidase (LOX) silencing by Lentiviral transfection on the breast cancer cell line MDA-MB-231. Methods; Base on the sequence of LOX, lentiviral RNA interference vector was designed and chemically synthesized, and then transfected into the breast cancer cell line MDA-MB-231. The expression of LOX mRNA and protein was detected by Real-time fluorogenic quantitative PCR (realtime PCR) and Western blot. Results: After the stable transfection, the expression of LOXmRNA and protein was significantly inhibited. Conclusion: Stable transfection of lentiviral vector interference LOX-RNAi-LV can significantly inhibit the expression of LOX mRNA and protein.关键词
乳腺癌/赖氨酰氧化酶/RNA干扰Key words
breast cancer/lysyl oxidase (LOX)/RNA interference分类
医药卫生引用本文复制引用
李景涛,刘剑仑,韦薇,杨华伟..慢病毒载体介导RNA干扰对乳腺癌MDA-MB-231细胞LOX基因表达的影响[J].广西医科大学学报,2011,28(6):834-836,3.基金项目
广西科学研究与技术开发计划课题(No.桂科攻0816004-14) （No.桂科攻0816004-14）
