广西植物2011，Vol.31Issue(6)：725-729,5.DOI:10.3969/j.issn.1000-3142.2011.06.005
扎龙芦苇遗传多样性ISSR分析
Genetic diversity of Phragmites australis populations from Zhalong based on ISSR analysis
摘要
Abstract
The genetic diversity of 5 wild Phragmites australis populations from Zhalong was estimated using inter-simple sequence repeat (ISSR) markers,using ten specific and stable primers selected from 75 primers. A total of 96 sites were generated,94 of which(97. 92%)were polymorphic. The data was analyzed by PopGene. The average percentage of polvmorphicloci of populations was 56. 46% and demonstrated high genetic diversity (the average value of Shannon's index (I) was 0. 4783). To some extent,both gene flow and genetic diversity were present among popula-tions (Nm was 0. 9249,average Nei's index was 0. 3150,and average Gst was 0. 3509). The gene proportion of diversity reached 64. 91% within population but was 35. 09% among populations. This demonstrated that genetic variation of P. Australis populations was mainly within populations. According to cluster analysis,the populations were classified into two large groups:the populations(K,T and J)grouped together,whereas populations (M and Z) grouped together. These results would supplement the information for protection and utilization of P. Australis and also provide further data for the study of genetic variation and species differentiation of P. Australis.关键词
芦苇/居群遗传结构/遗传变异Key words
Phragmites australis/population genetic structure/genetic variation分类
农业科技引用本文复制引用
杨晓杰,佟守正,程加春,李娜..扎龙芦苇遗传多样性ISSR分析[J].广西植物,2011,31(6):725-729,5.基金项目
国家自然科学基金(40971053) （40971053）
