广西植物2011，Vol.31Issue(6)：806-812,7.DOI:10.3969/j.issn.1000-3142.2011.06.018
蛇苔过氧化物酶基因的序列分析与功能预测
Sequence analysis and function predication of poroxidase from Conocephalum conicum
摘要
Abstract
Peroxidases is widespread in biological world. It plays an important role in the cell redox metabolism. In order to furher study the characteristics and functions of Conocephalum conicum poroxidase, we performed bioinformat-ics analysis of its homology,subellular location,tertiary structure,function and so on. The results showed that the sequence had a complete open read frame of about 1 050 bp and encodes 349 amino acids,and the sequence had similar POD with that of Arabidopsis thaliana(the similarity beween them is 71%). This sequence was predicated to be C. Conicum poroxidase which belonged to the poroxidase super family,and R68、F71、H72、P170、H200 and D276 to be the C. Conicum POD's active sites in which the four groups of disulfide bonds:C4 and C122,C74 and C79X128 and C328、C207 and C239 played a very important role in maintaining the protein's function,natural conformation and stability. This study would set an important foundation for the study of the interaction mechanisms and functions of the C. Conicum poroxidase.关键词
过氧化物酶/蛇苔/序列分析/功能预测Key words
peroxidases/Conocephalum conicum/sequence analysis/function predication分类
农业科技引用本文复制引用
李茹兰,边春象,阮期平,彭正松,姜立春,刘美..蛇苔过氧化物酶基因的序列分析与功能预测[J].广西植物,2011,31(6):806-812,7.基金项目
绵阳师范学院校级科研基金(2011A05) （2011A05）
