广西植物2011，Vol.31Issue(6)：827-831,5.DOI:10.3969/j.issn.1000-3142.2011.06.022
巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究
Primary study on suspension cell culture of Epimediun wushanense
摘要
Abstract
The technological system of cell suspension culture by the flavonoids content of E. Wushanense was established through studing the effect of inoculation concentration, hormone combination, sugar concentration and various medias. The result showed that the suspension culture optimum medium of E. Wushanense was B5 medium supplemented with 1.0 mg· L-1 2,4-D and 0. 2 mg·L-1 6-BA,the sugar concentration was 4. 0% and the optimum inoculation concentration was 2 g per 30 mL. The highest content of the total flavonoids appeared on the medium.关键词
巫山淫羊藿/细胞悬浮培养/黄酮类化合物Key words
Epimediun wushanense/suspension cell culture/flavonoids分类
农业科技引用本文复制引用
韩素菊,黎云祥..巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究[J].广西植物,2011,31(6):827-831,5.基金项目
四川省教育厅重点项目(09ZX011):四川省科技厅应用基础研究项目(2008JY0158) （09ZX011）
四川省重点实验室开放基金项目(XNYB09-04) （XNYB09-04）
