广西植物2012，Vol.32Issue(1)：113-117,5.DOI:10.3969/j.issn.1000-3142.2012.01.022
尾巨桉HMGR基因的克隆及表达分析
Molecular Cloning and expression analysis of 3-hydroxy-3-methylglutaryl-CoA Reductase Gene from Eucalyptus urophylla × E.grandis
摘要
Abstract
The gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR)was cloned by Rapid Amplification of cDNA Ends (RACE)method, the full-length EuHMGR cDNA was 1955 bp, containing a 1 560 bp open reading frame, encoding a peptide of 519 amino acids. Bioinformatics analysis indicated EuHMGR contained two HMG-CoA binding motifs and two NADP(H)binding motifs;and it had a 3-D structure with"V"homology-based on modeling, containing N-domain,S-domain and L-domain. There was a 319 bp intron in EuHMGR gene genomic DNA sequence compared with the cDNA sequence. Tissue expression profile analysis by Real-Time quantitative PCR indicated EuHMGR expression was the highest in stem,followed by leaf and no expression in root. The paper would supply some information to the function analysis and genetic transformation of EuHMGR from Eucalyptus urophylla × E. grandis.关键词
尾巨桉/HMGR/生物信息学/内含子/组织特异性表达Key words
Eucalyptus urophyla×E. grandis/ HMGR/ bioinformatics/ intron/ tissue specific expression分类
农业科技引用本文复制引用
蒋春,张华玲,彭江..尾巨桉HMGR基因的克隆及表达分析[J].广西植物,2012,32(1):113-117,5.基金项目
重庆市自然科学基金重点项目(2009BA1004) （2009BA1004）
三峡库区生态环境教育部重点实验室开放基金(EF200609) （EF200609）
