广西植物2012，Vol.32Issue(1)：118-123,6.DOI:10.3969/j.issn.1000-3142.2012.01.023
茅苍术愈伤组织诱导及其细胞悬浮培养研究
Research on callus induction and cell suspension culture of Atractylodes lancea
摘要
Abstract
Effect of different plant growth regulator on petiole,leaf and rhizome callus induction was studied by orthogonal design in this paper. It showed that petiole,leaf and rhizome callus induction rate were 99. 05%、83. 5% and 71. 5% in their optimum culture conditions respectively. And Petiole was the most easy to form callus. There was a significant promotion effect of 2,4-D on the callus induction. The optimal composition for petiole callus induction was 0. 4 mg· L-1NAA、4. 0 mg · L-1 2,4-D and 0. 4 mg · L-1 KT;and induction rate reached 99. 0% when petiole being cultured for 20 d. In addition, when cell from petiole callus was cultured for 18 d,the maximum yields of cell bio-mass, polysaccharide and atractylodin were 9. 07 g · L-1,15、 68 mg · L-1 and 19. 62 ug · L-1 respectively.关键词
茅苍术/愈伤组织/细胞悬浮培养Key words
Atractylodes lancea/ callus/ cell suspension culture分类
农业科技引用本文复制引用
陶金华,濮雪莲,江曙..茅苍术愈伤组织诱导及其细胞悬浮培养研究[J].广西植物,2012,32(1):118-123,6.基金项目
国家自然科学基金(81102743) （81102743）
江苏省高校自然科学基础研究项目(07KJD360164) （07KJD360164）
江苏省中医药局项目(HL07076) （HL07076）
