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VC磷酸酯的HPLC分析

张会轻 袁立国 王淑华 王云霞

河北工业科技2011,Vol.28Issue(6):364-365,371,3.
河北工业科技2011,Vol.28Issue(6):364-365,371,3.

VC磷酸酯的HPLC分析

HPLC analysis of L-ascorbic acid-2-phosphate

张会轻 1袁立国 1王淑华 1王云霞1

作者信息

  • 1. 河北维尔康制药有限公司,河北石家庄 050031
  • 折叠

摘要

Abstract

A phase high performance liquid chromatographic method has been developed for the determination of L-ascorbic acid-2-phosphate. Agilent Eclipse XDB C18(250 mm×4.0 mm, 5 μm) column is used and the mobile phase is the mixture of methanol- 0.1 mol/L phosphoricacid two hydrogen potassium buffer solution (35 : 65, V/V). The flow rate is 1. 0 mL/min, and the detection wavelength is 254 nm. The linear range is 0. 01~0. 10 mg/mL, and the related coefficient is 0. 999 9, the average recovery rate of L-ascorbic acid-2-phosphate in the samples is 98. 24%.

关键词

VC磷酸酯/高效液相色谱法/VC

Key words

L-ascorbic acid-2-phosphate/HPLC/vitamin C

分类

化学化工

引用本文复制引用

张会轻,袁立国,王淑华,王云霞..VC磷酸酯的HPLC分析[J].河北工业科技,2011,28(6):364-365,371,3.

河北工业科技

OACSTPCD

1008-1534

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