河北科技大学学报2011，Vol.32Issue(5)：426-430,481,6.
胶束电动毛细管色谱法测定何首乌中的二苯乙烯苷、大黄素和大黄酚
Determination of stilbene glycoside, emodin and chrysophanol in radix polygoni muliflori by micellar electrokinetic capillary chromatography
摘要
Abstract
A new assay is developed by use of micellar electrokinetic capillary chromatography to determine stilbene glycoside, emodin and chrysophanol in radix polygoni muliflori. The factors affecting separation are optimized. The running buffer contains 25 mmol/L borax, 40 mmol/L sodium dodecyl sulfate and 10% ethanol, and its pH value is adjusted to 9. 5. Under the optimum conditions, the linear dynamic range is 2.0~l 000 mg/L for stilbene glycoside, 5. 2~260 mg/L for emodin and 4. 5 ~225 mg/L for chrysophanol. The limits of detection are 1. 10, 0. 29 and 0. 43 mg/L for stilbene glycoside, emodin and chrysophanol, respectively. The average recoveries are 98. 8%, 98. 8% and 100. 7% for stilbene glycoside, emodin and chrysophanol. The assay meets the determination requirement of stilbene glycoside, emodin and chrysophanol in radix polygoni muliflori , and can be applied to quality control of the raw material of radix polygoni muliflori.关键词
胶束电动毛细管色谱/何首乌/二苯乙烯苷/大黄酚/大黄素Key words
micellar electrokinetic capillary chromatography/ radix polygoni muliflori/ stilbene glycoside/ chrysophanol/ emodin分类
医药卫生引用本文复制引用
吴娴,陈冠华,王坤,石杰,武传芹..胶束电动毛细管色谱法测定何首乌中的二苯乙烯苷、大黄素和大黄酚[J].河北科技大学学报,2011,32(5):426-430,481,6.基金项目
教育部高等学校博士学科点专项科研基金资助项目(20093227110010) （20093227110010）
江苏大学科研基金资助项目(08JDG001) （08JDG001）
江苏省高校优势学科建设工程资助项目 （）
