河北科技大学学报2012，Vol.33Issue(1)：36-39,4.
金莲花中荭草苷在兔体内组织中的分布特征研究
Distribution of orientin from Trollius Chinensis in tissue of rabbits
摘要
Abstract
A method using HPLC is established to determine orientin in tissue of rabbits and to study the characteristic of the orientin when injected into veins. After orientin is injected into veins, tissue samples are collected at time sharing. The rabbit is put to death by bloodletting, then the heart, liver, brain, kidney, lung and spleen are taken out to prepare homogenate tissue. HPLC is used for determination of orientin in tissue of rabbits. Theory of statistical moment is used to account AUC of each tissue sample. Endogenous material in tissue does not interfere in determination of orientin. In amplitude of 0. 050 0～50. 0 mg/L, mass concentration of orientin and peak area have a good linear relation, and the quantitation of lower limit is 0. 050 0 mg/L. The RSD of precision,accuracy and plus sample recovery are all less than 15%. The AUC order of each tissue is kidney>liver>lung>spleen>heart>brian. After log transition of AUC data, normality test, homogeneity test for variance and analysis of variance are conducted. The result shows AUC of each tissue have significant difference(P<0. 05). Orientin distribute in dominating tissue of rabbits is obviously different.关键词
荭草苷/分布/HPLCKey words
orientin/ distribution/ HPLC分类
医药卫生引用本文复制引用
陈凤平,曲彩红,安芳,王书华..金莲花中荭草苷在兔体内组织中的分布特征研究[J].河北科技大学学报,2012,33(1):36-39,4.基金项目
河北省教育厅科学研究计划项目(2008101) （2008101）
