河北医学2012，Vol.18Issue(2)：143-147,5.DOI:10.3969/j.issn.1006-6233.2012.02.001
白细胞介素1受体拮抗剂对大鼠肾间质纤维化干预作用的研究
The Effect of Interleukin-1 Receptor Antagonist on Renal Interstitial Fibrosis in Rats
摘要
Abstract
Objective; To observe the influence of interleukin-1 receptor antagonist (IL-1ra) on renal interstitial fibrosis and renal function induced by unilateral ureteral obstruction. Method; 72 rats were randomly divided into 3 groups; sham operation (SOR) group, unilateral ureteral ligation( UUO) group and IL -1ra treatment! Tre) group. The unilateral ureteral obstruction operation was performed with the UUO group and the Tre group, the same operation was performed with SOR group, but ureter wasn't tied up. The rats in Tre group were injected 10% IL-lra solution per day, while rats in the other two groups were injected equal volume of sterile water. At the lth, 3th, 7th and 14th days after operation there were 6 rats of each group were killed, their blood and obstructed kidneys were collected for creatinine and urea nitrogen detection, HE and Masson staining and immunohistochemistry, which would reveal the level of renal interstitial fibrosis and renal function. Result; Along with obstruction time extension, The UUO group and the Tre group kidney mesenchymal fibrosis's degree were higher than the SOR group , and the tendency which after the kidneyfunction has worsen first, changed for the better (P<0.05) ;The Tre group kidney mesenchymal fibrosis degree has reduced, the kidney function change for the better tendency also compares the UUO group to be obvious , The difference has statistics significance from the 7 th day (P<0.05). Conclusion: IL-1 ra has therapeutic effect on renal interstitial fibrosis, which may be associated with inhibition of interleukin-1.关键词
白细胞介素1受体拮抗剂/UUO模型/肾间质纤维化Key words
Interleukin-1 receptor antagonist/UUO model/Renal interstitial fibrosis引用本文复制引用
江燕,张旭,张翠薇,高岑,荣智利,马跃荣..白细胞介素1受体拮抗剂对大鼠肾间质纤维化干预作用的研究[J].河北医学,2012,18(2):143-147,5.基金项目
四川省教育厅自然科学资助项目(07ZA038) （07ZA038）
