郑州大学学报（医学版）2011，Vol.46Issue(6)：852-855,4.
尘肺病噬菌体单链抗体库的构建及鉴定
Construction and identification of human phage single-chain antibody library for pneumoconiosis
摘要
Abstract
Aim: To construct a human pneumoconiosis phage single-chain antibody ( ScFv) library with diversity. Methods:Total RNA was extracted from the peripheral blood lymphocytes from the patients with pneumoconiosis, VH and VL genes were amplified by semi-nested PCR and were assembled to form ScFv by Linker and cloned into phagemid pCANTAB-5E, and then transformed into E. Coli TGI to construct a human peumoconiosis phage scFv antibody library. Re-SUltS:VH gene families and VL gene families were successfully amplified. A large human antibody library containing 2.4 × 107 pfu/mL clones was created after rescuing the recombinant phagemids from the transformed E. Coli TGI cells. Conclusion : A human ScFv library is successfully constructed finally.关键词
尘肺/噬菌体单链抗体库/单链抗体Key words
pneumoconiosis/ phage single-chain antibody library /ScFv分类
医药卫生引用本文复制引用
翟伟,郝长付,高彬,姚武..尘肺病噬菌体单链抗体库的构建及鉴定[J].郑州大学学报（医学版）,2011,46(6):852-855,4.基金项目
国家自然科学基金资助项目 81072278 （）
