郑州大学学报（医学版）2011，Vol.46Issue(6)：856-858,3.
Hela细胞雄激素受体基因外显子1的甲基化检测
Methylation analysis of androgen receptor exon-1 in Hela cells
摘要
Abstract
Aim:To investigate the methylation level of the androgen receptor( AR) exon-1 in Hela cells. Methods: Methylation analysis of the AR exon-1 was performed by bisulfite genomic sequencing( BGS) and combined bisulfite restriction analysis( COBRA). The sequencing data were analyzed by BiQ Analyzer. Results: At relatively high conversion rate ( >98% ) , the methylation ratio of the AR exon-1 was still more than 96. 2% . Except for the eighth CpG, all other CpGs under investigations were fully or highly methylated. The results of COBRA were in accordance with that of BGS. Conclusion : DNA methylation may involve in the inactivation of AR gene in Hela cells.关键词
雄激素受体/DNA甲基化/Hela细胞Key words
androgen receptor/ DNA methylation/ Hela cell line分类
医药卫生引用本文复制引用
李笑竹,赵贵森,岳阳阳,翟仙敦,艾红伟,薛小琦..Hela细胞雄激素受体基因外显子1的甲基化检测[J].郑州大学学报（医学版）,2011,46(6):856-858,3.基金项目
河南省重点科技攻关基金资助项目 082102310097 （）
河南科技大学科研基金资助项目 2007QN013 （）
