郑州大学学报（医学版）2011，Vol.46Issue(6)：904-906,3.
机动车尾气有机提取物对HepG2细胞的遗传毒性
Genotoxicity effects of organic extract of vehicle exhaust on HepG2 cells in vitro
郭海玲 1夏向群 2杨海燕3
作者信息
- 1. 北京中医药大学东直门医院护理部 北京100700
- 2. 河南省疾病预防控制中心环境卫生科 郑州450016
- 3. 郑州大学公共卫生学院流行病学教研室 郑州450001
- 折叠
摘要
Abstract
Aim: To explore the genotoxicity of organic extract of vehicle exhaust on HepG2 cells in vitro. Methods: Cytokinesis-block micronucleus assay and comet assay were used to evaluate the genotoxicity of organic extract of vehicle exhaust (2.5,5.0 and 10.0 mg/L) on HepG2 cells. The solvent control group and positive control group were used as control. Results: There were significant differences in frequency of cytokinesis-block micronucleus and Olive tail moment a-mong five groups( F = 18. 608 and 9.525 ,P <0. 001). A significant increased frequency of cytokinesis-block micronucleus was observed in both 5. 0 and 10.0 mg/L dose group compared with the solvent control group( P < 0. 05 ) . A significant increased Olive tail moment was observed in three dose groups compared with the solvent control group(P <0.05 ). Conclusion :The organic extract of vehicle exhaust has a distinct genotoxicity on HepG2 cells in vitro.关键词
机动车尾气/双核细胞微核/彗星试验/遗传毒性Key words
vehicle exhaust/cytokinesis-block micronucleus/comet assay/ genotoxicity分类
医药卫生引用本文复制引用
郭海玲,夏向群,杨海燕..机动车尾气有机提取物对HepG2细胞的遗传毒性[J].郑州大学学报（医学版）,2011,46(6):904-906,3.
