郑州大学学报（医学版）2011，Vol.46Issue(6)：912-914,3.
亳菊中木犀草素和木犀草苷含量的反相高效液相色谱法测定
Determination of luteolin-7-O-glucoside and luteolin in Chrysanthemum morifolium by RP-HPLC
王存良 1李远哲2
作者信息
- 1. 郑州大学第一附属医院药剂科 郑州450052
- 2. 河南工业大学生物制药系 郑州450001
- 折叠
摘要
Abstract
Aim:To develop a RP-HPLC method for the determination of luteolin-7-0-glucoside and luteolin in Chrysanthemum morifolium. Methods:RP-HPLC was carried out on a Purospher star Scienhome C18 (4.6 mm ×200 mm,5 μm) at 30 ℃ , at a flow rate of 1 mL/min. The detection wavelength was 350 no. Results:The linearity of luteolin-7-0-glucoside was found in the range of 0. 004 8 ~ 0. 038 4 |xg, Y = 10 000 000X - 6 650, r = 0. 999 9, the average recovery was 104. 9% .and RSD was 1.64% . The linearity of luteolin was found in the range of 0. 032 ~ 0. 256μg, Y = 8 000 OOOX -20 581,r = 0.999 9, the average recovery was 101.7% ,and RSD was 1.68% . Conclusion:This method is reliable,accurate and suitable for the determination of luteolin-7-O-glucoside and luteolin in Chrysanthemum morifolium, and plays an important role in the quality control of Chrysanthemum morifolium.关键词
反相高效液相色谱/毫菊/木犀草素/木犀草苷Key words
HPLC/Chrysanthemum morifolium/luteolin/luteolin-7-0-glucoside分类
医药卫生引用本文复制引用
王存良,李远哲..亳菊中木犀草素和木犀草苷含量的反相高效液相色谱法测定[J].郑州大学学报（医学版）,2011,46(6):912-914,3.
