郑州大学学报（医学版）2012，Vol.47Issue(1)：10-13,4.DOI:10.3969/j.issn.1671-6825.2012.01.004
结核分枝杆菌CFP21抗原的HLA-A*0201/*03限制性细胞毒T淋巴细胞表位预测
Prediction of HLA-A0201/03-restricted cytotoxic T lymphocyte epitopes in Mycobacterium tuberculosis CFP21 antigen
摘要
Abstract
To explore the HLA-A * 0201/ * 03 cytotoxic T lymphocyte( CTL ) epitopes from Mycobacterium tuberculosis CFP21. Methods:The online database was applied to predict the HLA-A * 03 restricted natural epitope peptide based on the HLA-A * 0201 candidate peptides of CFP21 antigen, and modified epitopes were obtained by amino acid replacement. T2A3 cell line was used to determine the binding affinity of the candidate peptides, and the candidate peptides were selected for subsequent cytotoxicity assay. ReSU Its: Four native epitopes and 3 modified epitopes were obtained. The binding affinity of the modified epitope pl34-lY2L, pl34-lY2L9L and the native pl34 towards HLA-A * 03 molecule was relatively high. Cytotoxicity assay showed that both the modified epitope pl34-lY2L9L and the native peptide pl34 could induce potent CTL response, and pl34-induced CTLs could potently lyse the peptide-loaded target cells. Conclusion: pl34 ( AVADHVAAV ) could be a broad spectrum CTL epitope towards HLA-A * 0201/ * 03.关键词
结核分枝杆菌/CFP21/表位Key words
Mycobacterium tuberculosis /CFP21 /epitope分类
医药卫生引用本文复制引用
翟文杰,翟明霞,吕虹,祁元明,高艳锋..结核分枝杆菌CFP21抗原的HLA-A*0201/*03限制性细胞毒T淋巴细胞表位预测[J].郑州大学学报（医学版）,2012,47(1):10-13,4.基金项目
国家自然科学基金资助项目 30872381 （）
30901362 （）
