郑州大学学报（医学版）2012，Vol.47Issue(1)：14-16,3.DOI:10.3969/j.issn.1671-6825.2012.01.005
甘蓝型油菜二酰甘油酰基转移酶基因微藻真核表达载体的构建
Construction of eukaryotic expression vector for microalga using cloned DGAT genes from Brassica napus
摘要
Abstract
To construct the diacylglycerol acyltransferase( DGAT ) eukaryotic expression vector for microalga. Methods: DGAT1 and DGAT2 were obtained from Brassica napus through RT-PCR and then cloned into vector pMD18-T Simple. The cDNA fragments indentified correctly by sequencing were subcloned into an eukaryotic expression vector to construct pSV40DGATl/CaMVBar and pSV40DGAT2/CaMVBar. Results: The cloned cDNA sequences were 1 512 and 1 026 bp with hemology of 99% and 98% respectively, and were utilized for constructing eukaryotic expression vectors pSV40DGATl/CaMVBar and pSV40DGAT2/CaMVBar. Conclusion: The two eukaryotic expression vectors for microalga have been successfully constructed.关键词
甘蓝型油菜/二酰甘油酰基转移酶/微藻/真核表达载体Key words
Brassica napus/diacylglycerol acyltransferase/microalga/eukaryotic expression vector分类
生物科学引用本文复制引用
张楠楠,潘卫东,郑国庭,李靓,崔玉琳,秦松,薛乐勋..甘蓝型油菜二酰甘油酰基转移酶基因微藻真核表达载体的构建[J].郑州大学学报（医学版）,2012,47(1):14-16,3.基金项目
国家自然科学基金资助项目 30700014 （）
科技部国际科技合作基金资助项目 2007DFA01240 （）
