郑州大学学报（医学版）2012，Vol.47Issue(2)：153-155,3.DOI:10.3969/j.issn.1671-6825.2012.02.005
伊马替尼敏感及耐药K562细胞P-糖蛋白、缺氧诱导因子和鞘氨醇激酶表达的比较
Comparison of expressions of P-glycoprotein,HIF-1α,and SPK between K562 and K562/Ima cells
摘要
Abstract
Aim:To compare the expressions of P-glycoprotein( P-gp ),HIF-1α,and SPK between chronic myeloid leukemia cell line K562 and imatinib-resistance K562( K562/Ima )cell line. Methods: K562 and K562/Ima cells were treated with different doses of imatinib for 48 h, and MTT was used to calculate the resistance fold. Western blot, RT-PCR and TLC were used to detect the expressions of P-gp, HIF-1α and SPK in K562 and K562/Ima cells, respectively. Results: The results revealed K562/Ima cells showed 24 fold resistance to imatinib compared with K562 cells. The expressions of P-gp, SPK and HIF-1α were up-regulated in K562/Ima cells. Conclusion: It is concluded that the resistance of K562/Ima cells is related to the high expressions of P-gp,SPK and HIF-1α.关键词
伊马替尼/K562/P-糖蛋白/鞘氨醇激酶/缺氧诱导因子1αKey words
imatinib/K562/P-glycoprotein/SPK/HIF-1 α分类
医药卫生引用本文复制引用
许严伟,赵小利,王英,苗润宏,张庆林,杜钢军..伊马替尼敏感及耐药K562细胞P-糖蛋白、缺氧诱导因子和鞘氨醇激酶表达的比较[J].郑州大学学报（医学版）,2012,47(2):153-155,3.基金项目
国家自然科学基金资助项目 30472082 （）
