河南农业大学学报2011，Vol.45Issue(6)：657-661,5.
用EST-SSR标记评价21份黄瓜种质的遗传多样性
Analysis of genetic diversity of 21 cucumber germplasms using EST-SSR markers
摘要
Abstract
In this study, 15 EST-SSR primer pairs were used to analyze the genetic diversity of 21 cu-cumber germplasms containing various ecotypes, I. E. North China ecotype, South China ecotype, Eu-ropean greenhouse ecotype and wild species. A total of 65 alleles were detected with a mean of 4. 33 , and polymorphism information content (PIC) varied from 0. 177 to 0.748 with a mean of 0.470. Out of the 15 primer pairs, 12 primer pairs were high or medium polymorphism. Based on the EST-SSR da-ta, clustering analysis using UPGMA method revealed that 21 cucumber germplasms could be divided into two broad groups, and foreign germplasms were well separated from domestic germplasms. Also, domestic germplasms could be subdivided into 3 subgroups based on their ecotypes. Principal coordi-nate analysis (PCoA) revealed a similarity to clustering analysis.关键词
黄瓜/EST-SSR标记/遗传多样性Key words
Cucumis sativus/ EST-SSR marker/ genetic diversity分类
农业科技引用本文复制引用
刘俊青,李佩芳,胡建斌,李建吾,陶翠..用EST-SSR标记评价21份黄瓜种质的遗传多样性[J].河南农业大学学报,2011,45(6):657-661,5.基金项目
河南省科技攻关重点项目(102102110037) （102102110037）
河南省教育厅自然科学研究计划项目(2009A210010) （2009A210010）
河南农业大学博士科研启动基金项目(30400247) （30400247）
