河南农业大学学报2011，Vol.45Issue(6)：662-667,6.
鸡传染性法氏囊病毒在DF-1细胞系上繁殖特性研究
Research on the breeding characters of IBDV on DF-1cell line
摘要
Abstract
Study on adaption and propagation conditions of IBDV HQ strain propagated in DF-1 cells suggests that vero cells are not suitable for IBDV HQ strain to propagate but DF-1 cells are. In flask op-timization test proved that DMEM/F12 containing 2% serum, pH 7.0,1: 100 inoculation concentration of IBDV and 36 h cultivation for IBDV harvesting after its inoculation were the optimal conditions whe-IBDV was propagated on DF-1, the titers are more than 8. 5 mL-1 and 10 times as high as those propa-gated in vero cells. The titers of cell suspension and supernatant on DF-1 cell are basically the same after inoculation for 24 ~ 36 h. The peak consumption of sugar came before that of titers and decreased abruptly after the top. The titers of IBDV were the highest when the consumption of sugar was near ze-ro.关键词
传染性法氏囊病毒/DF-1细胞/病毒感染复数/糖耗/毒价Key words
infectious bursal disease virus (IBDV) / DF-1 cell/ multiplicity of infection ( MOI) / sugar consumption/ virus titer分类
农业科技引用本文复制引用
王永生,周欣,杨霞,陈陆,王川庆,王泽霖..鸡传染性法氏囊病毒在DF-1细胞系上繁殖特性研究[J].河南农业大学学报,2011,45(6):662-667,6.基金项目
农业科技成果转化资金项目(2008GB2D000182) （2008GB2D000182）
