化工进展2011，Vol.30Issue(11)：2517-2522,6.
Lipozyme TL 100L的固定化及其催化合成蔗糖-6-乙酯
Immobilization of Lipozyme TL 100 L and catalytic synthesis of sucrose-6-acetate
杨绪娥 1郑璞 1孙志浩 1倪晔1
作者信息
- 1. 江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122
- 折叠
摘要
Abstract
Immobilization of Lipozyme TL 100 L on resin was realized by adsorption,and its application in sucrose-6-acetate（S-6-a）synthesis was then investigated.The results showed that nonpolar macroporous resin of HZ-802 was the best carrier among 7 kinds of resins by comparing both protein adsorptivity and transesterification capacity.The S-6-a yield was attained more than 80% in the condition of immobilization temperature 30 ℃,adsorption capacity between 50.47～58.1 mg/g resin,and immobilized enzyme relative water content under 5%.Disposing Lipozyme TL 100 L with 2-propanol at the volume ratio of 0.5∶1,the S-6-a yield of the immobilized enzyme reached 95.4%.And the yield still remained 34.2% when the immobilized enzyme was repeated 8 times.The transesterification followed Ping-Pong Bi Bi mechanism by the studies of apparent michaelis constant Km′ at different substrate concentrations.Activation energy of the immobilized enzyme by adsorption on HZ-802 was reduced 6.47 kJ/mol compared with the commercial Lipozyme TLIM.关键词
脂肪酶/树脂/固定化/转酯反应/蔗糖-6-乙酯/动力学Key words
lipase/resin/immobilization/transesterification/sucrose-6-acetate/kinetics分类
生物科学引用本文复制引用
杨绪娥,郑璞,孙志浩,倪晔..Lipozyme TL 100L的固定化及其催化合成蔗糖-6-乙酯[J].化工进展,2011,30(11):2517-2522,6.
