化工进展2011，Vol.30Issue(12)：2714-2718,5.
一株烟腈水解酶菌株的筛选及其催化特性初步研究
Isolation and catalytic characteristics of a 3-cyanopyridinehydrolyzing strain
摘要
Abstract
Rhodococcus sp.CCZU10-1 has been isolated from soil samples with high nitrilase activity against 3-cyanopyridine using the enrichment culture technique.The preferred carbon sources,nitrogen sources,and inducer were glucose（10 g/L）,a composite of peptone（10 g/L）and yeast extract（5 g/L）,and ε-caprolactam（2.0 mmol/L）,respectively.The optimal reaction temperature and pH for 3-cyanopyridine-hydrolyzing activity were 30 ℃ and pH 7.0,respectively.Using fed-batch method,a total of 927 mmol/L nicotinic acid has been accumulated in the reaction mixture after the 10th feed.关键词
烟腈/烟酸/Rhodococcussp.CCZU10-1/筛选/催化特性Key words
3-cyanopyridine/nicotinic acid/Rhodococcus sp.CCZU10-1/screening/catalytic characteristics分类
生物科学引用本文复制引用
何玉财,丁亮,周琼,张跃,王龙耀,王利群,高大舟,郑明,赵希岳,卢彬..一株烟腈水解酶菌株的筛选及其催化特性初步研究[J].化工进展,2011,30(12):2714-2718,5.基金项目
国家自然科学基金 （）
江苏省社会发展项目 （）
常州市生物医药科技专项 （）
常州大学人才引进基金（ZMF10020077）及常州大学大学生创新基金（10-化-A-11）项目 （）
