化工进展2011，Vol.30Issue(12)：2719-2723,5.
非离子表面活性剂对木质纤维素酶解的促进作用
Enhanced enzymatic hydrolysis of lignocelluloses by non-ionic surfactant
摘要
Abstract
Low specific activity,high enzymatic loading and easy acitivity losing of cellulose enzyme have become bottlenecks for bioprocessing the lignocellulosic biomass into bioenergy and bioproducts.In this work,non-ionic surfactants were added to reduce the enzymatic loadings.Its effect on the hydrolysis of lignocelluloses was also studied.Experimental results showed that the non-ionic surfactant helped to enhance the hydrolysis of lignocellulose with 0.5% addition.With the addition of Tween-80 and PEG6000,Glucose produced from enzymatic hydrolysis of atmospheric glycerol pretreated wheat straw increased approximately 20%.The non-ionic surfactant also had a positive impact on the enzymatic hydrolysis of cellulosic biomass with less lignin and even no lignin.The glucose outputs from the filter paper and avicel increased almost by 90% and 70%—80%,respectively.The addition of non-ionic surfactants provided a higher diffusion coefficient k for enzymatic hydrolysis system,increased reaction efficiency in heterogeneous phase,and made a significantly lower Km in enzymatic reaction dynamics,therefore remarkably enhanced the affinity of substrates on cellulase.关键词
非离子表面活性剂/木质纤维素/纤维素酶/酶解Key words
non-ionic surfactant/lignocellulose/cellulase/enzymatic hydrolysis分类
能源科技引用本文复制引用
孙付保,娄秀平,洪嘉鹏,顾琴琴,陈晓旭,蔡宇杰,廖祥儒..非离子表面活性剂对木质纤维素酶解的促进作用[J].化工进展,2011,30(12):2719-2723,5.基金项目
国家自然科学基金青年科学基金项目 （）
