化工学报2012，Vol.63Issue(1)：223-229,7.DOI:10.3969/j.issn.0438-1157.2012.01.031
基于能量代谢分析的S-腺苷蛋氨酸和谷胱甘肽联合高产方法
Strategy for enhanced co-production of S-adenosylmethionine and glutathione by Candida utilis based on energy metabolic analysis
摘要
Abstract
The concentrations of intracellular co-factors, such as ATP, ADP, NADH and NAD+ vital for energy metabolism within Candida utilis CCTCC M 209298 were measured by HPLC. Based on the levels of co-factors, especially ATP during batch cultivation of C. Utilis CCTCC M 209298, we developed a novel culture strategy of L-cysteine addition followed by ATP accumulation (21 h) instead of cell growth ceased (15 h) for further enhanced co-production of S-adenosylmethionine and glutathione using energy metabolic analysis. Subsequently, we combined this strategy with high-cell-density culture of C. Utilis CCTCC M 209298, and both high biomass and high co-production of S-adenosylmethionine and glutathione were achieved. The culture strategy under the guidance of ATP concentration was proved to be efficient in terms of full utilization of intracellular residual ATP, together with improvement of co-production of S-adenosylmethionine and glutathione, and the co-production of S-adenosylmethionine and glutathione attained 584. 7 mg·L-1 and 1004. 4 mg·L-1 from batch and fed-batch fermentation, which were 27. 1% and 26. 4% higher than the controls, respectively. The results presented in this study provide a feasible strategy for optimization of fermentative processes for enhancing the co-production of analogous useful chemicals biosynthesized by consuming energy.关键词
S-腺苷蛋氨酸/谷胱甘肽/ATP/产朊假丝酵母/联产发酵Key words
S-adenosylmethionine/glutathione/ATP/Candida utilis/co-production分类
化学化工引用本文复制引用
王玉磊,卫功元,邵娜,聂敏..基于能量代谢分析的S-腺苷蛋氨酸和谷胱甘肽联合高产方法[J].化工学报,2012,63(1):223-229,7.基金项目
国家自然科学基金项目(20906065) （20906065）
江苏省属高校自然科学研究项目(09KJB530009). （09KJB530009）
