化工学报2012，Vol.63Issue(3)：887-893,7.DOI:10.3969/j.issn.0438-1157.2012.03.030
产磷脂酶菌株的筛选鉴定及其应用
Screening and identification of phospholipase-producing strains and their applications
摘要
Abstract
Using soybean phospholipids as substrates, one strain which expressed phospholipase was screened out from the oil-rich soil sample by preliminary screening of plate cultivation and re-screening of shake flask fermentation. According to the morphological characteristics, physiochemical properties, and 16S rRNA sequences, the strain BIT-18 was identified as Pseudomonas fluorescens. Synthetic phosphatecholine (l-palmitoyl-2-oleoyl phosphatecholine) was used as substrate, then the fatty acid compositions of hydrolyzates were analyzed by gas chromatography, and the phospholipase from BIT-18 was determined as phospholipase B. This enzyme could not tolerate a high temperature. The optimal conditions for the enzymatic reaction were 251 and pH 6. 5, and low concentrations of metal ions were good for trigging its reaction. The phospholipase B from BIT-18 was used as catalyst for the degumming of soybean oil in a self-made batch reactor. The optimal conditions were phospholipase B dosage of 500 U · kg-1 and 2% water, and reaction at 40℃ , pH 4. 7 for 6 h. The residual phosphorus content of degummed oil decreased from 90. 1 mg · kg-1 to 4. 6 mg · kg-1, with the degumming rate as high as 94. 9%, which showed a good application potential in the degumming of vegetable oils.关键词
磷脂酶B/筛选/鉴定/酶法脱胶/荧光假单胞菌Key words
phospholipase B/screening/identification/enzymatic degumming/Pseudomonas fluorescens分类
生物科学引用本文复制引用
姜芳燕,王金梅,戴大章,李春..产磷脂酶菌株的筛选鉴定及其应用[J].化工学报,2012,63(3):887-893,7.基金项目
国家水体污染控制与治理科技重大专项资助课题(2008ZX07207-003-03). （2008ZX07207-003-03）
