环境工程学报2011，Vol.5Issue(10)：2385-2390,6.
中原油田石油污染土壤原位生物修复技术实验研究
Experimental study on in-situ bioremediation technique for oil contaminated soil in Zhongyuan Oil Field
摘要
Abstract
The degradation microbial communities which were lab selective enrichment cultured from oil polluted soil of Zhongyuan Oil Field could fast grow using crude oil as carbon source.These microbial communities binding with Ryegrass and Alfalfa were used in experiments on combined in-situ bioremediation of oil contaminated soil.The experimental area was divided into five： microbial communities with Ryegrass,microbial communities with Alfalfa,only microbial communities,only Ryegrass and blank.After 99 d bioremediation,the cumulative degradation rate of petroleum hydrocarbon could reach 43.27% to 67.38%,which were much higher in the two areas of microbial communities with Ryegrass or Alfalfa.Whereas the cumulative degradation rate of blank area was lower than 2.5%.Those results showed that the selected microbial communities could obviously degrade the oil polluted soil in central plains oil field.The experimental results indicated that the inoculated degradation microbial communities generally need 1～3 d adaptive phase then being active,few nutriment such as soluble salt,NO-3,Cl-,etc could infiltrate into the lower soil layer（50 cm）.The nitrogen fixation of Alfalfa roots may cause the distinct increase of NH＋4.In addition,some factors containing temperature,water,oxygen,nutriment and minigeoenvirorment were optimized in the experiments.关键词
生物修复/石油污染/土壤/原位修复/降解Key words
bioremediation/oil contamination/soil/in-situ remediation/degradation分类
资源环境引用本文复制引用
陈立,张发旺,刘少玉,何泽,张胜..中原油田石油污染土壤原位生物修复技术实验研究[J].环境工程学报,2011,5(10):2385-2390,6.基金项目
科技部国际合作重点项目 （）
河南省国土资源厅基金项目 （）
中国地质科学院水文地质环境地质研究所基本科研业务费项目 （）
