环境工程学报2012，Vol.6Issue(2)：658-664,7.
纤维素高效降解菌NU-H的诱变选育及降解特性
Mutation breeding and degradation and characteristics of an efficient cellulose-decomposing strain NU-H
摘要
Abstract
A strain（NU-H） with high cellulase production was selected from Penicillium oxalicum D1 by ultraviolet mutation,nitrous acid mutation and the compound mutation of ultraviolet and nitrous acid.As compared with the original strain,the carboxymethyl cellulase（CMCase） and filter paper enzyme activities of NU-H increased by 69.08% and 75.28%,respectively.A significant increase of xylanase activity and reducing sugar yield was also observed.The single factor experiments and the resulting orthogonal experiments were designed to deter mine the optimal enzyme hydrolysis conditions of straw using the cellulase produced by NU-H.Results showed that the optimal enzyme hydrolysis conditions were as follows： temperature of 50℃,time of 28 h,enzyme concentration of 60%,substrate concentration of 3%,pH of 4.8.Under these conditions,the yield of reducing sugar was up to 23.25%.关键词
草酸青霉/纤维素酶/诱变/酶水解Key words
Penicillium oxalicum/cellulase/mutation/enzyme hydrolysis分类
资源环境引用本文复制引用
石文卿,陶能国,刘跃进..纤维素高效降解菌NU-H的诱变选育及降解特性[J].环境工程学报,2012,6(2):658-664,7.基金项目
国家自然科学基金资助项目 （）
国际科学基金IFS资助项目 （）
湖南省自然科学基金资助项目 （）
