黑龙江八一农垦大学学报2011，Vol.23Issue(5)：46-49,75,5.
利用梯度PCR和降落PCR扩增CIRP基因的比较
Comparison of the Amplification of CIRP Genes with Touch Down PCR and Gradient PCR
摘要
Abstract
To extract the total RNA from rats,then to target CIRP genes by touch down PCR（TD-PCR） and gradient PCR（G-PCR） with the popular reaction system and the reaction system content low primer.The results indicated that TD-PCR was efficient in amplifying the CIRP gene from rats and it was more specific than G-PCR.TD-PCR with the same reaction system has higher specificity than G-PCR.It can efficiently improve specificity of PCR and be used to clone gene fragments which are difficult to clone by the G-PCR method.关键词
降落PCR/梯度PCR/退火温度/CIRP基因Key words
touch down PCR/gradient PCR/annealing temperature/CIRP gene分类
生物科学引用本文复制引用
李玉恒,李士泽,赵明慧,赵紫阳,李峥,袁雪,李昌盛,林增,胡婧,李俭..利用梯度PCR和降落PCR扩增CIRP基因的比较[J].黑龙江八一农垦大学学报,2011,23(5):46-49,75,5.基金项目
农业部948重点项目 （）
2011年黑龙江省研究生创新科研项目 （）
