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开菲尔粒中产球菌样细菌素乳酸菌的分离与鉴定

王颖 桑文彦 姚笛 高玉荣 于洋

黑龙江八一农垦大学学报2011,Vol.23Issue(5):50-53,4.
黑龙江八一农垦大学学报2011,Vol.23Issue(5):50-53,4.

开菲尔粒中产球菌样细菌素乳酸菌的分离与鉴定

The separation and Identification of lactobacillus to secrete Like-Coccidia bacteriocin in Kefir grains

王颖 1桑文彦 2姚笛 1高玉荣 1于洋1

作者信息

  • 1. 黑龙江八一农垦大学食品学院,大庆163319
  • 2. 大庆市第三医院
  • 折叠

摘要

Abstract

To screen lactic acid bacteria of bacteriostasis using MRS selection culture medium.The agar diffusion method with Oxford cup methord was adopted,combining physical and chemical resistance of the characteristics identification,antibacterial spectrum determination colony morphology and characteristics,physiological and biochemical experiment,and the berger's manual identification were exercised.The physiological and biochemical reaction was lactic acid.The bacteria colony morphology and characteristics were Coccidia and have inhibition for most of the gram negative bacteria,this strain was lactic acid milk aureus the diacetyl lactic acid subspecies.Identification and separation results was in the bacteriocins coccoid lactic acid bacteria,and the bacteriocin was the like-protein peptide.

关键词

开菲尔粒/球菌样细菌素/抑菌机理/分离/鉴定

Key words

kefir grains/neisseria gonorrhoeae-like bacteriocin/bacteriostatic mechanism/separation/identification

分类

农业科技

引用本文复制引用

王颖,桑文彦,姚笛,高玉荣,于洋..开菲尔粒中产球菌样细菌素乳酸菌的分离与鉴定[J].黑龙江八一农垦大学学报,2011,23(5):50-53,4.

基金项目

黑龙江省青年骨干计划项目 ()

黑龙江八一农垦大学学报

1002-2090

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