黑龙江八一农垦大学学报2011，Vol.23Issue(6)：44-48,5.
响应面法优化枯草芽孢杆菌BS-1101原生质体制备条件
Optimization of Protoplast Formation Conditions of Bacillus Subtilis BS-1101 by Using Response Surface Methodology
姚建波 1吴志军 1陈志宝1
作者信息
- 1. 黑龙江八一农垦大学生命科学技术学院,大庆163319
- 折叠
摘要
Abstract
The central composite design combined with response surface methodology was employed to optimize the protoplast formation conditions of Bacillus subtilis BS-1101.The critical parameters,such as lysozyme concentration,temperature,and contact time which affects the formation of protoplast of the Bacillus subtilis BS-1101 were studied using a novel technique named response surface methodology.Second-order multiple regression analysis was used to build a predicted model of the combined effects of independent variables（enzyme concentration,temperature and effect time） for maximum the yield of protoplast of Bacillus subtilis BS-1101.The optimal parameters to obtain the highest protoplast yield were as followed： temperature was 37.27 ℃,effect time was 88.8min,enzyme concentration was 5.152 mg·mL-1.The average experimental protoplast yield under the optimum conditions was found to be 77.02%,which agreed with the predicted value.The proposed method showed high degree of reproducibility.The central composite design was employed to optimize the protoplast formation conditions of Bacillus subtilis BS-1101.关键词
枯草芽孢菌/原生质体/响应面法Key words
bacillus subtilis/protoplast/response surface methodology分类
农业科技引用本文复制引用
姚建波,吴志军,陈志宝..响应面法优化枯草芽孢杆菌BS-1101原生质体制备条件[J].黑龙江八一农垦大学学报,2011,23(6):44-48,5.
