黑龙江农业科学Issue(2):1-5,5.
龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基(LGW-GS)鉴定
LMW-GS Identification at Gli-A1/GluA3 Loci in NILs of Wheat Variety Longmai 19
摘要
Abstract
In order to identify LMW-GS at Glu-A3 locus linked with Gli-A1 locus in common wheat variety Longmai 19(L-19,HMW-GS 2*,7+9,2+12)and a pair of NILs(L-19-613 and L-19-626)at Gli-A1 locus with genetic background of Longmai 19 with HMW-GS 5+10,the materials that L-19,L-19-626 and L-19-613,F2 progeny of L-19/L-19-626 were assayed by a set STS markers of LMW-GS at Glu-A3 locus.The results showed that Glu-A3e amplified specific band in L-19-626 type materials with gliadin Gli-A1m.in L-19 and L-19-613 type materials without gliadin Gli-A1m,Glu-A3c amplified specific band.These results indicated the Gli-A1m at Gli-A1 locus was linked with LGW-GS Glu-A3e at Glu-A3 locus and the undesignated gliadin at Gli-A1 locus in L-19-613 and L-19 were linked with LGW-GS Glu-A3c at Glu-A3 locus.It was possible that quality difference between NILs L-19-626 and L-19-613 was due to LMW-GS gene difference of Glu-A3e and Glu-A3c at Glu-A3 locus.关键词
低分子麦谷蛋白亚基/近等基因系/STS标记Key words
LMW-GS/NILs/STS marker分类
农业科技引用本文复制引用
宋维富,赵海滨,张延滨,李集临,姜焕焕,丁琦,宋庆杰..龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基(LGW-GS)鉴定[J].黑龙江农业科学,2012,(2):1-5,5.基金项目
国家自然科学基金资助项目 ()