哈尔滨商业大学学报（自然科学版）2011，Vol.27Issue(5)：654-658,5.
β-葡聚糖酶对β-1,3-葡聚糖的最佳酶解条件
Study of β - 1,3 - glucan by β - glucanase in optimum of enzyme condition
摘要
Abstract
This paper studied on enzymolysis of β - 1,3 - glucan in the optimum of enzyme condition by β - glucanase. Effects of substrate concentration, enzyme amount, reaction temperature and reaction pH on yield of reducing sugar content were studied. On this basis, orthogonal were designed to investigate the enzymatic hydrolysis of time. The results showed that the optimum substrate was 1.4 mg/mL, enzymatic hydrolysis was 14 mg/mL, and temperature was 55 ℃, pH 5.5 for 10 minutes. In the condition, a maximum of reducing sugar hydrolysates yield was 95.30% .关键词
β-葡聚糖酶/β-1,3-葡聚糖/酶解作用Key words
β - glucanase/ β - 1,3- glucan/ enzymatic hydrolysates分类
医药卫生引用本文复制引用
张岩,高世勇,季宇彬..β-葡聚糖酶对β-1,3-葡聚糖的最佳酶解条件[J].哈尔滨商业大学学报（自然科学版）,2011,27(5):654-658,5.基金项目
黑龙江省自然科学基金重大项目(ZJY0504) （ZJY0504）
教育部博士点基金项目(20070240081). （20070240081）
