哈尔滨商业大学学报（自然科学版）2012，Vol.28Issue(1)：8-13,6.
藻红蛋白调控ΔΨm诱导人乳腺癌MCF-7细胞凋亡
Study on phycoerythrin induces MCF -7 apoptosis by controlling variation of Δψm
高世勇 1王静 2季宇彬1
作者信息
- 1. 哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076
- 2. 国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076
- 折叠
摘要
Abstract
To investigate the effect of phycoerythrin induces MCF - 7 apoptosis by controlling variation of △Ψm· The inhibition of phycoerythrin on MCF -7 cells detected by MTT assay. Hoechst33258 staining was observed under fluorescence microscope role of phycoerythrin gly-coprotein MCF - 7 cell morphology. Apoptosis rate of MCF - 7 by phycoerythrin ( FCM). Effect of phycoerythrin on variation of △Ψm in MCF -7 cells observed by CLSM. The phycoerythrin can restrain the growth of tumor cell MCF -7. The IC50 was 81. 60 (μg/L for the method of MTT. The morphology of cell was observed by invert microscope in cell collapsed, break to pieces. Hoechst33258 staining showed obvious appearance of apoptosis: nuclear condensation and fragmentation, chromatin condensation, and apoptotic body formation. 40, 80, 160 (μg/L of phycoerythrin on MCF -7 the apoptosis rate of MCF -7 by phycoerythrin was 25. 18% , 55.45% , 83.94%. Result of phycoerythrin on variation of △Ψm in MCF -7 cells observed by CLSM. 24.40 ± 0.21, 20.45 ± 0.24, 19. 77 ± 0. 27 (FI). Phycoerythrin induces MCF -7 apoptosis by controlling variation of △Ψm.关键词
藻红蛋白/人乳腺癌MCF -7细胞/细胞凋亡/线粒体膜电位Key words
phycoerythrin/human breast cancer MCF -7 cells/apoptosis/△Ψm.分类
医药卫生引用本文复制引用
高世勇,王静,季宇彬..藻红蛋白调控ΔΨm诱导人乳腺癌MCF-7细胞凋亡[J].哈尔滨商业大学学报（自然科学版）,2012,28(1):8-13,6.
