湖南大学学报（自然科学版）2011，Vol.38Issue(11)：66-69,4.
拟南芥Rad23原核重组蛋白构建和活性检测
Purification of Arabidopsis Rad23 Recombinant Protein in Engineering Bacteria and Preparation of Its Polyclonal Antibody
摘要
Abstract
Rad23 gene which participates in ubiquitin/26S proteasome pathway in Arabidopsis, is known to play important roles in plant development and responses to hormone. In order to study its function and molecular mechanism, an expression vector pCold-Rad23 was constructed and transformed into E. Coli BL21. Then the recombinant fusion protein was induced by IPTG. The results of SDS-PAGE have shown that Rad23 fusion protein existed in a soluble form with a relative molecular mass 94 ku, which is fit for the molecular mass supposed from gene coding frame. Finally, the Rad23 recombinant fusion protein was used as an antigen to prepare polyclonal antiserum in mice. The western blot results have shown that the polyclonal antiserum has high specificity and can react to the native protein expressed in different tissues of Arabidopsis.关键词
蛋白/泛素/26S蛋白酶体/原核表达/抗体Key words
protein/ubiquitin (Ub)/26S proteasome/prokaryotic expression/antibodies分类
生物科学引用本文复制引用
李秀山,刘斌,赵李剑,唐东英,赵小英,刘选明..拟南芥Rad23原核重组蛋白构建和活性检测[J].湖南大学学报（自然科学版）,2011,38(11):66-69,4.基金项目
国家自然科学基金资助项目(30770200) （30770200）
