华南农业大学学报2012，Vol.33Issue(1)：64-68,81,6.
红树林固氮菌和解磷菌的分离及对秋茄苗的促生效果
Isolation of Nitrogen-Fixing Bacteria and Phosphate-Solubilizing Bacteria from the Rhizosphere of Mangrove Plants and Their Enhancement to the Growth of Kandelia candel Seedlings
摘要
Abstract
Fifty nine isolates of nitrogen-fixing bacteria and phosphate-solubilizing bacteria were isolated based on the characteristics of morphology and growth of the bacteria on the selective growth media from the rhizosphere of mangrove plants including Bruguiera gymnorhiza, B. Sexangula, Kandelia candel, Rhizophora stylosa, Sonneratia apetala and S. Caseolaris in Zhanjiang and Zhuhai, Guangdong Province and Dongzhai harbor, Hainan Island. Among them 7 isolates of nitrogen-fixing bacteria and 8 isolates of phosphate-solubilizing bacteria were screened as propagule inoculants of K. Candel in a series of potted experiments to examine their capability to promote the growth of plants. The results showed that the inoculation with a single isolate of phosphate-solubilizing bacteria He4# resulted in a significant increase in plant height, root mass and total biomass at the rate of 58.4% , 132. 1% and 133. 2% , respectively. The enhancement of isolate He4# on the plant growth is more significant than that of an alien isolate (Bacillus lichenciformis). The root biomass and total biomass of K. Candel inoculated He4# increased by 72. 7% and 90. 2% than those inoculated B. Lichenciformis. All pure inoculations with 7 different isolates of nitrogen-fixing bacteria showed positive response to the plant growth, and the inoculation with isolateNgqq-R14 resulted in the maximum increase in plant height, stem diameter, stem dry mass, root dry mass and total biomass at the rate of 38. 36% , 16. 19% , 75. 46% , 51. 55% and 59. 31% , respectively. Dual inoculations with 7 mixtures of one isolate of nitrogen-fixing bacteria and one isolate of phos-phate-solubilizing bacteria could result in synergistic effect on the growth of the seedlings of K. Candel, and the increase of plant height, stem dry mass, root dry mass and total biomass were 21. 1% -69. 4% , 70. 7% -271. 3% , 27. 1% - 111. 3% and 66. 9% - 123. 8% respectively. It can be concluded that u-sing mixed cultures of microorganisms is advantageous over pure cultures, and the inoculation of mangroves with phosphate-solubilizing bacteria and nitrogen-fixing bacteria will promote the growth of mangrove plants.关键词
红树林/固氮菌/解磷菌/分离/促生菌Key words
mangrove/ nitrogen-fixing bacteria/ phosphate-solubilizing bacteria/ isolation/ plant growth promoting bacteria分类
农业科技引用本文复制引用
何雪香,李玫,廖宝文..红树林固氮菌和解磷菌的分离及对秋茄苗的促生效果[J].华南农业大学学报,2012,33(1):64-68,81,6.基金项目
"十一五"国家林业科技支撑计划专题(2009BADB2B0401) （2009BADB2B0401）
国家林业局"948"项目(2004-4-14) （2004-4-14）
