华南农业大学学报2012，Vol.33Issue(1)：92-95,4.
框镜鲤维氏气单胞菌CY0806株鞭毛flaA基因的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of Flagellum flaA Gene of Aeromonas veronii CY0806 from Cyprinus carpio
摘要
Abstract
The flaA gene was amplified from genome template of Aeromonas veronii CY0806 of Cyprinus carpio by PCR. Homogeneous analysis was carried out by comparing the sequencing results with other strains of Aeromonas and bioinformatic analysis was done on the protein coded by flaA. The results showed that flaA Gene with a length of 915 bp, coding 304 amino acids, was obtained; phylogenetic tree analysis results showed that it was on the same branch with other strains of Aeromonas. Bioinformatic analysis results showed that the relative molecular mass of the protein was 32 098. 66, pi 5. 57, half-life 30 h, unstability index 32. 31, fat index 81. 05 whereas there was no signal sequence and transmembrane domain. Results of secondary structural analysis showed that the main secondary structures were ot-helix and /3-sheet. At the same time the tertiary structure was also predicted by homology modeling.关键词
框镜鲤/维氏气单胞菌/flaA基因/生物信息学Key words
Cyprinus carpio/ Aeromonas veronii/ flaA gene/ bioinformatic analysis分类
农业科技引用本文复制引用
单晓枫,吴同垒,孟庆峰,王伟利,钱爱东..框镜鲤维氏气单胞菌CY0806株鞭毛flaA基因的克隆与生物信息学分析[J].华南农业大学学报,2012,33(1):92-95,4.基金项目
国家科技支撑计划项目(2010BAD04B01) （2010BAD04B01）
吉林省科技支撑计划重点项目(20080218) （20080218）
吉林农业大学青年启动基金 （）
