华南农业大学学报2012，Vol.33Issue(1)：102-105,4.
狂犬病病毒HEP-Flury株N基因的修饰及原核表达
Modification and Prokaryotic Expression of Nucleoprotein Gene of Rabies Virus Strain HEP-Flury
摘要
Abstract
Using bioinformatics analysis, a rich antigenic determinant area in HEP-Flury nucleoprotein sequence was selected, and the codons were modified to cater the codon usage bias in Escherichia coli, and the restriction enzyme sequences were inserted in upstream and downstream. The modified gene was synthesized and cloned into prokaryotic expression vector pET-32a( + ). The recombinant plasimd was designated pET-32-NP. pET-32-NP wasl75 transformed to E. Coli BL21 ( DE3)pLysS, and the recombinant protein was expressed after IPTG induction. SDS-PAGE analysis showed that the expressed protein had a relative molecular mass of 34 000. Western-blotting results also revealed that the expressed protein could be recognized specifically by mouse anti-His monoclonal antibody and mouse anti-RV polyclonal antibody.关键词
狂犬病病毒/核蛋白/原核表达Key words
rabies virus/ nucleoprotein/ prokaryotic expression分类
农业科技引用本文复制引用
张良,黄永亮,官培英,王怡飞,徐晓娟,吴晓薇,郭霄峰..狂犬病病毒HEP-Flury株N基因的修饰及原核表达[J].华南农业大学学报,2012,33(1):102-105,4.基金项目
国家自然科学基金(30871876) （30871876）
公益性行业(农业)科研专项(201103032) （农业）
