河南农业科学2011,Vol.40Issue(10):38-40,3.
小麦叶绿体DNA提取方法研究
Study on Extraction Method of Wheat Chloroplast DNA
摘要
Abstract
An improved high-salt low-pH method was used to extract chloroplast DNA from leaf tissues of wheat cultivar Chinese spring. The agrose gel electrophoresis and PCR amplification showed that wheat chloroplast DNA of high purity (OD260/280 = 1. 89, OD260/230 - 1. 23) was obtained. The DNA was suitable to be used as template in PCR, gene amplification and enzyme digestion.关键词
小麦/叶绿体DNA/提取方法/高盐—低pH法Key words
Wheat/ Chloroplast DNA/ Extraction method/ High-salt low-pH method分类
农业科技引用本文复制引用
侯典云,马占强,徐虹,郭蔼光..小麦叶绿体DNA提取方法研究[J].河南农业科学,2011,40(10):38-40,3.基金项目
教育部新世纪优秀人才支持计划(02240601) (02240601)
河南科技大学人才科学研究基金(09001428) (09001428)
河南科技大学青年科学基金(2010QN0004) (2010QN0004)
河南科技大学实验技术开发基金项目(SY1011043) (SY1011043)