河南农业科学2011，Vol.40Issue(10)：52-55,4.
转基因烟草抗性外植体培育的研究
Research on Cultivation of Transgenic Tobacco's Explant
摘要
Abstract
This research was to study the influence of different cultivation methods,hormones and antibiotics on explant differentiation of transgenic tobacco. There were five different culture media for the research,including MS+lmg/L 6-BA + O. lmg/L NAA for co-cultivation, MS+lmg/L 6-BA+O. 1mg/L NAA+500mg/L Carb for sterilization cultivation, MS + 100mg/L Kan for screening, MS + 80 mg/L Kan+300mg/L Carb for subculture, and MS +0. Lmg/L NAA + 300 mg/L Carb for root induction. With these mediums the explant of transgenic tobacco K326 could differentiate rapidly, which laid the basis for further cultivation of the whole plants of transgenic tobacco K326.关键词
转基因烟草/外植体/培养基/转化/共培养Key words
Transgenic tobacco/ Explant/ Culture medium/ Transformation/ Co-cultivation分类
农业科技引用本文复制引用
沙琰琰,刘品,史团省,张志杰,赵青..转基因烟草抗性外植体培育的研究[J].河南农业科学,2011,40(10):52-55,4.基金项目
国家自然科学基金项目(30771301) （30771301）
教育部留学回国人员科研启动基金项目 （）
