河南农业科学2011，Vol.40Issue(10)：126-130,5.
猪口蹄疫O型合成肽疫苗抗原多肽的串联表达及活性鉴定
Tandem Expression and Activity Analysis of Antigen Peptide of Swine Foot and Mouth Disease (Type O) Synthetic Peptide Vaccine
摘要
Abstract
In order to get the recombination protein which contained antigen peptide of synthetic peptide vaccine, two-copy gene was synthesized using E. coli optimal codons according to the ami no acid sequence of antigen peptide. The recombinant expression vector pET32a-P2 was construc ted by cloning two-copy gene of antigen peptide into the prokaryotic expression plasmid pET32a. The BL2KDE3) was transformed with pET32a-P2,and the protein corresponding to P2 gene was expressed after induction with IPTG. SDS-PAGE and Western-blot with the product of bacterial culture induction with IPTG showed that the molecular mass of the protein was approximately 27 kD,which was identified by positive sera of FMDV serotype O. The soluble analysis showed that the recombination protein was almost solubly expressed. The protein was purified by Ni NTA resins and assayed by ELISA,and the results showed that it demonstrated high purity and activity. So the recombination protein which contained antigen peptide of synthetic peptide vaccine was expressed solubly and efficiently in E. coli, and the purified protein could be developed as diagnosis antigen or vaccine.关键词
猪口蹄疫/合成肽疫苗/抗原多肽/串联表达/蛋白纯化/活性鉴定Key words
Swine foot and mouth disease/ Synthetic peptide vaccine/ Antigen peptide/ Tandem expression/ Protein purification/ Activity analysis分类
农业科技引用本文复制引用
卢清侠,郭军庆,郝慧芳,杨苏珍,邢广旭,杨继飞,王世红,张改平..猪口蹄疫O型合成肽疫苗抗原多肽的串联表达及活性鉴定[J].河南农业科学,2011,40(10):126-130,5.基金项目
国家"863"计划(2007AA100606) （2007AA100606）
中国博士后科学基金面上资助项目(20110491000) （20110491000）
