河南农业科学2012，Vol.41Issue(1)：106-109,4.
怀地黄基因片段及其中转座酶基因的克隆与序列分析
Cloning and Sequencing of One DNA Fragment and Its Internal Transposase Gene in Rehmannia glutinosa.f.hueichingensis (Chan et Sehih)Hsiao
摘要
Abstract
A pair of primers were designed according to the base information of the promoter PNZ-IP in Pharbitis nil (L. ) Ching using software primer 3. 0. Five DNA fragments of the genomic DNA in Rehmannia glutinosa. F. Hueichingensis (Chan et Sehih) Hsiao were amplified; one fragment (1 096 bp) was cloned by PCR. The result showed that it was one transposon-like protein gene sequence, including a complete ORF,450 bp in size and encoding one 150-amino acid trans-posase(alignment with some gene sequences in NCBI). Furthermore,the another pair of primers were designed according to the base information of the ORF and the ORF was generated completely by PCR. The results will provide significant references for cloning the functional genes from Rehmannia glutinosa. F. Hueichingensis (Chan et Sehih) Hsiao and their structural and functional studies in the future.关键词
怀地黄/转座酶基因/基因克隆/开放阅读框/序列分析Key words
Rehmannia glutinosa f. Hueichingensis(Chan et Sehih)Hsiao/transposase gene/gene cloning/ORF/sequencing分类
农业科技引用本文复制引用
周延清,姚换灵,段红英,周春娥,张永华,陈艳梅,张喻..怀地黄基因片段及其中转座酶基因的克隆与序列分析[J].河南农业科学,2012,41(1):106-109,4.基金项目
河南省基础与前沿技术研究计划项目(092300410009) （092300410009）
河南省教育厅自然科学研究计划项目(2011A180022) （2011A180022）
