河南农业科学2012，Vol.41Issue(1)：137-141,5.
豫西地区PRRSV新近流行株ORF5基因变异及Nsp2基因特征分析
Genetic Variations of the ORF5 and Nsp2 Genes of the Prevalent PRRSV Isolates from Western Henan Province
摘要
Abstract
To determine the genetic variations of the ORF5 and Nsp 2 genes of prevalent PRRSV isolates from western Henan,the complete ORF 5 gene and subsequence for Nsp 2 gene of PRRSV were amplified by RT-PCR from lung samples of swine. A total of 21 complete ORF 5 and subsequence for Nsp 2 sequences were successfully amplified from 26 case samples. Homology for 21 complete ORF 5 gene and amino acids sequences are 96. 5% - 100% or 97. 5% -100%,respectively. Compared to the reference strains JXA1 ,MLV,and VR-2332,the homology of gene were 96. 5% -98. 8% ,87. 4% -89. 1%,and 87. 7% - 89. 4%. Subsequence for Nsp2 sequence were amplified from positive samples and compared to other published sequences, two point deletions and 90 bp were deleted. It indicates that the prevalent PRRSV isolates from western Henan belong to Northern American genotype. Our study provides some basis for further controlling PRRS in this area.关键词
猪繁殖与呼吸综合征病毒/流行株/ORF 5基因/Nsp2基因/变异分析Key words
PRRSV/prevalent strain/ORF5 gene/Nsp2 gene/variation analysis分类
农业科技引用本文复制引用
张明亮,张春杰,程相朝,赵星灿,李银聚,吴庭才,李正,杨宁宁..豫西地区PRRSV新近流行株ORF5基因变异及Nsp2基因特征分析[J].河南农业科学,2012,41(1):137-141,5.基金项目
洛阳市科技攻关项目(0801048A) （0801048A）
