河南农业科学2012，Vol.41Issue(2)：126-128,135,4.
蝴蝶兰叶片诱导类原球茎初探
Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis
摘要
Abstract
There are serious browning and low induction rate when the protocorn-like body (PLB) was induced from leaves of Phalaenopsis amabilis. Using the grem free leaf of Phalaenopsis ama-bilis H807 as materials, the effects of anti-browning materials(citric acid, ascorbic acid and activated charcoal)and cytokinins (6-BA and TDZ) on the protocorn-like body induction was investigated. The results indicated that the rank of anti-browning only reached the level 1 with 70 mg/L ascorbic acid,which was best to prevent in vitro leaves from browning. The protocorn-like body induction rate reached 75. 0% with 3 mg/L TDZ. On the medium with 1/2MS+70 mg/L ascorbic acid,hormone combination of 3 mg/L TDZ+1 mg/L NAA was the best for PLB induction.关键词
蝴蝶兰/叶片/褐化/类原球茎/诱导率Key words
Phalaenopsis amabilis/ leaf/ browning/ the protocorn-like body/ induction rate分类
农业科技引用本文复制引用
张玉,李艳敏,张和臣,王利民,王慧娟,张鸽香..蝴蝶兰叶片诱导类原球茎初探[J].河南农业科学,2012,41(2):126-128,135,4.基金项目
河南省重点科技攻关项目(092102110127) （092102110127）
